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KMID : 0545120180280050816
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 5 p.816 ~ p.820
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Effects of flaC Mutation on Stringent Response-Mediated Bacterial Growth, Toxin Production, and Motility in Vibrio cholerae
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Kim Hwa-Young
Yu Sang-Mi
Jeong Sang-Chul
Yoon Sang-Sun
Oh Young-Taek
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Abstract
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The stringent response (SR), which is activated by accumulation of (p)ppGpp under conditions of growth-inhibiting stresses, plays an important role on growth and virulence in Vibrio cholerae. Herein, we carried out a genome-wide screen using transposon random mutagenesis to identify genes controlled by SR in a (p)ppGpp-overproducing mutant strain. One of the identified SR target genes was flaC encoding flagellin. Genetic studies using flaC and SR mutants demonstrated that FlaC was involved in bacterial growth, toxin production, and normal flagellum function under conditions of high (p)ppGpp levels, suggesting FlaC plays an important role in SR-induced pathogenicity in V. cholerae.
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KEYWORD
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Vibrio cholerae, stringent response, (p)ppGpp, flaC, cholera toxin, motility
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